Effect of nitric oxide on biochemical and antioxidant properties of pomegranate fruit cv. Shishe-kab during cold storage

Pomegranate is a subtropical fruit that is widely consumed as fresh fruit and juice, however, its postharvest life is limited mainly due to storage disorders. The aim of this study was to determine the effect of nitric oxide (NO) on antioxidant activity and quality attributes of pomegranate fruit. The fruits were dipped for two minutes into different NO concentrations (0, 30, 100, 300 or 1000 µM). Then, the fruits were stored in cold room at 5 °C and RH 85%. After 90 days storage, changes in chilling injury, electrolyte leakage, titratable acidity (TA), total soluble solids (TSS), pH, antioxidant activity and total anthocyanin were evaluated. The results showed that 1000 µM NO application resulted in significant decrease in electrolyte leakage and TSS and maintained antioxidant activity and total anthocyanin in pomegranate fruit. However, no significant effect of NO treatment was observed on TA and chilling injury index. In conclusion, postharvest application of NO may be a promising method for maintaining quality of pomegranate fruit during cold storage.